Green Light Suppresses Cell Growth but Enhances Photosynthetic Rate and MIB Biosynthesis in PE-Containing Pseudanabaena
Supplementary Material
a State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.
b Sino-Danish College, Sino-Danish Center for Education and Research, University of Chinese Academy of Sciences, Beijing 100190, China.
c School of Civil Engineering, Chang’an University, Xi’an 710054, China.
d University of Chinese Academy of Sciences, Beijing 100049, China.
* Corresponding to: Ming Su (mingsu@rcees.ac.cn), Min Yang (yangmin@rcees.ac.cn)
Figures and/or tables are provided below as the supplementary evidences to the main text.
The cell density (A) and MIB concentration (B) of MIB-producing Pseudanabaena cinerea under different light color.
The cellular MIB yield of MIB-producing Pseudanabaena cinerea under different light color (A) and culture time (B).
The expression level (expressed in log10FPKM) of genes encoding phycobilisomes (PE：cpeD, cpeU, and cpeY; PC: cpcA; APC: apcE; chlorophyll: chlG, chlH, and chlN), photosystem II (psbC), photosystem I (psaB and psaF), photoelectron transfer pigments (petD) of MIB-producing Pseudanabaena cinerea under green light and red light.
The detailed biosynthesis pathway of carbon fixation in MIB-producing Pseudanabaena cinerea. Pink symbols represented the up-regulated genes under green light compared to red light.
The up-regulated and down-regulated pathways under green light compared to red light at pathway levels 2. Pink and blue symbols represented the up-regulated and down-regulated pathways by green light compared to red light.
The up-regulated and down-regulated pathways under green light compared to red light. The Metabolism, environmental information processing, genetic information processing, and cell growth and death are the physiological functions at pathway level 1.
The MIB yield per chl a production of MIB-producing Pseudanabaena cinerea under different light color.
| Pathway level3 | Pathway level1 | Gene Num | Richfactor | p-value |
|---|---|---|---|---|
| Photosynthesis-antenna proteins | Metabolism | 15 | 0.5172 | 0.0000 |
| Ribosome | Genetic Information Processing | 19 | 0.3585 | 0.0000 |
| Carbon fixation | Metabolism | 8 | 0.5333 | 0.0000 |
| Photosynthesis | Metabolism | 17 | 0.2982 | 0.0000 |
| Glycolysis / Gluconeogenesis | Metabolism | 10 | 0.3846 | 0.0001 |
| RNA degradation | Genetic Information Processing | 8 | 0.3478 | 0.0015 |
| Pentose phosphate pathway | Metabolism | 6 | 0.3529 | 0.0054 |
| Glyoxylate and dicarboxylate metabolism | Metabolism | 6 | 0.3333 | 0.0074 |
| Citrate cycle (TCA cycle) | Metabolism | 4 | 0.3333 | 0.0287 |
| Pyruvate metabolism | Metabolism | 6 | 0.2400 | 0.0383 |
| Cysteine and methionine metabolism | Metabolism | 6 | 0.1875 | 0.1069 |
| Bacterial secretion system | Environmental Information Processing | 4 | 0.2667 | 0.0618 |
| Starch and sucrose metabolism | Metabolism | 4 | 0.2500 | 0.0759 |
| Biotin metabolism | Metabolism | 3 | 0.2308 | 0.1452 |
| Sulfur metabolism | Metabolism | 4 | 0.2353 | 0.0915 |
| Protein export | Genetic Information Processing | 3 | 0.2000 | 0.1988 |
| Sulfur relay system | Genetic Information Processing | 3 | 0.2000 | 0.1988 |
| Terpenoid backbone biosynthesis | Metabolism | 3 | 0.21429 | 0.1714 |
The enrichment pathways of Pseudanabaena cinerea in response to different light color.